Altogen Services offers generation of tetracycline inducible RNAi gene knockdown stable cell lines:

Inducible RNAi stable cell lines expressing shRNA construct targeting gene of interest are important gene expresion research tool. However, generation of tetracycline inducible RNAi stably-expressing cancer cell lines can be very laborious, expensive, and time-consuming.

Altogen Services offer generation of inducible stable cell lines based on client individual specifications. Our scientists bring years of extensive cell culture experience and expertise.

Service includes:

a) Cloning: plasmid DNA encoding antibiotic-resistant gene, synthesis and cloning of shRNA construct targeting gene of interest into plasmid DNA, final plasmid DNA amplification, Maxi-prep purification.

b) Stable cell line generation: transfection of plasmid DNA (at least 20 ug should be provided by client) into a cell line of choice, drug selection of clonal cells, generation of stably-expressing population of cells, validation of shRNA construct expression and RNAi gene silencing by real-time qRT-PCR (mRNA expression) or Western Blot (protein expression).

Western Blot Analysis

Altogen Services biochemically characterizes the protein or antibody via Western blot analysis under reducing or non-reducing electrophoresis conditions.

FACS Analysis

Fluorescence-activated cell sorting (FACS) analysis of the binding of antibody/Fc fusion protein to the customer’s cell line is performed.

Please let us know the details of your project, so that we can provide you with an accurate quote (contact e-mail: techserv@altogen.com) and timeline estimate.