Generation of Stably-expressing Cell Line in 28 Days:

Altogen Services uses its unique experience with over 100 cancer cell lines and highly efficient gene delivery technologies to produce stable clonal cells in just 28 days.

Altogen Services offers the following stable cell line generation services: protein-overexpressing cell lines, knockdown (RNAi) cell lines, and reporter stable cell lines (e.g., luciferase, GFP, RFP, and YFP).

Company scientists transform customer cell lines to stably express genes of interest or shRNA-encoding plasmid DNAs (plasmid DNA construct should encode antibiotic resistance gene to enable selection). Following clonal cell line selection, cells are expanded to develop a line that permanently expresses specific genes through gene incorporation into the cellular genome.

GPCRs: Generation of Stable Cell Lines Expressing GPCRs

Stable cell lines expressing proteins of interest are important life sciences research tools. However, the generation of stable cancer cell lines and primary cells can be very time-consuming, laborious, and expensive. Altogen Services offers the generation of clonal stable cell lines based on individual client specifications. Company scientists bring years of extensive cell culture experience and expertise, including 1) cloning (plasmid DNAs encoding antibiotic-resistant genes, cloning of GPCR constructs into plasmid DNAs, final plasmid DNA amplification, Maxi-prep purification) and 2) stable cell line generation [transfection of GPCR plasmid DNA (20 µg) into a cell line of choice, drug selection of clonal cells, generation of stable protein-expressing cells, final validation of construct expression by real-time qRT-PCR (i.e., mRNA expression) or Western blot (i.e., protein expression)]. Two clonal cell lines (one cryovial each) are delivered to the customer.

Altogen Services specializes in mammalian stable cell line generation and development of expression system to produce recombinant proteins. Company scientists have applied advanced technologies and cell biology techniques to rapidly develop clonal stable cell lines, capable of producing the protein of interest (or shRNA that targets the gene of interest) for in vitro and in vivo studies. Altogen Services is committed to offering the highest quality service at the lowest price for pharmaceutical, biotechnology, and academic institutions.

Please let us know the project details and we will be happy to provide an immediate price quote (contact e-mail: techserv@altogen.com). Experimental details allow accurate quotes and timeline estimates.