Altogen Services offers different types of siRNA products and services, including:

• Therapeutic small interference RNA (siRNA) development:

siRNA targeting fusion protein

siRNA against oncogenes

apoptosis-inducing siRNA

viral siRNA/shRNA/miRNA

microRNA (pre-mir, anti-mir, antagomir, onco-mir)

aptamer-based siRNA

• In Vivo siRNA: design, synthesis, modification, delivery
• siRNA or shRNA design (targeting gene of interest) and synthesis
• Experimental validation of siRNA gene silencing in vitro
• Quantitation of mRNA knockdown (qRT-PCR) and protein expression reduction (Western Blot)
• Cell line optimization of siRNA transfection
• Genome wide high-throughput screening (siRNA, miRNA, shRNA)
• Generation of knockdown RNAi cell lines: stably expessing shRNA targeting protein of interest
• Cloning: vector-based siRNA construction
• Generation of tetracycline inducible siRNA/shRNA plasmid DNA constructs
• siRNA screen: Identification of novel parts of signaling pathway
• High throughput screening: Synthetic siRNA and lentiviral shRNA libraries
• Transient (siRNA) and stable (shRNA) transfection service
• Minimizing siRNA off-target effects

Please contact us at following e-mail: techserv@altogen.com for project quotation and timeline estimate.