Altogen Services offers a number of RNAi (gene silencing) services, including transient RNAi (siRNA transfection), stable integration of shRNA-expressing construct (targeting protein of interest), and animal RNAi studies.

Our Standard RNAi services include:

• RNAi Screens
• Genome-wide siRNA and microRNA high-throughput screens
• In vivo siRNA delivery
• Gene targeting
• Generation of stable RNAi cell lines (stably expressing shRNA targeting gene of interest)
• RNAi reporter cell lines generation
• In Vitro Functional testing of siRNA-induced mRNA knockdown
• RNAi screen pathway analysis
• RNAi manufacturing
• Transient (siRNA) and stable (shRNA) transfection services
• siRNA, shRNA, miRNA design and synthesis
• siRNA and microRNA chemical modification to enhance stability in vivo
• PCR Cloning: shRNA-encoding vector construction
• Quantitation of siRNA/shRNA functional knockdown effect by qRT-PCR and Western Blot
• Altogen's tetracycline-inducible RNAi systems
• siRNA transfection optimization
• Reporter gene (RNAi) assays

Please let us know the details of your project, so that we can provide you with an accurate quote (contact e-mail: techserv@altogen.com) and timeline estimate.